Hydrophilic Interaction Chromatography: A Guide for Practitioners (BOK)

Bernard A. Olsen, Brian W. Pack

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Discover how to use HILIC to analyze and better understand polar compoundsAn increasingly popular analytical method, hydrophilic interaction chromatography (HILIC) has the ability to retain and separate polar compounds that are often difficult to analyze by reversed-phase high-performance liquid chromatography (HPLC) or other analytical methods. Offering a comprehensive review, this book enables readers to develop a fundamental understanding of how HILIC works and then apply that knowledge to develop and implement a variety of practical applications."Hydrophilic Interaction Chromatography" begins with discussions of HILIC retention mechanisms, stationary phases, and general method development. This sets the foundation for the book's extensive coverage of applications. The authors address unique separation challenges for bioanalytical, environmental, pharmaceutical, and biochemical applications. Moreover, there is a thorough discussion of HILIC in two-dimensional chromatography.With contributions from leading analytical scientists who have extensive experience in HILIC as well as HPLC, "Hydrophilic Interaction Chromatography" serves as a practical guide for researchers, featuring: Detailed examples of HILIC methods and development approachesThorough explanations of retention mechanisms and the impact of stationary phase and mobile phase properties on separationsStep-by-step guidance for developing efficient, sensitive, and robust HILIC methodsReferences to the primary literature at the end of each chapter"Hydrophilic Interaction Chromatography" is written for scientists who use or develop analytical methods for the separation of polar compounds. In particular, these researchers will discover how HILIC can be used to analyze and better understand the composition of pharmaceutical, bioanalytical, biochemical, chemical, food, and environmental samples.


Språk Engelsk Engelsk Innbinding Innbundet
Utgitt 2013 Forfatter Bernard A. Olsen, Brian W. Pack
ISBN 9781118054178
Antall sider 336 Dimensjoner 15cm x 25cm x 2,3cm
Vekt 590 gram Emner og form Chromatography