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This textbook aims to present the knowledge which is necessary for everyday HPLC measurements to be made, in a detailed and easy-to-follow manner. It contains a wealth of practical material and covers a wide range of subjects. Topics covered include principles, instrumentation, reverse-phase, ion-exchange, ion-pair, ion, size-exclusion and affinity chromatography, as well as analytical methods, preparative HPLC and enantiomeric separations. Appendices provide information on instrument testing, troubleshooting, column-packing.